Characterization of Leptospira isolates from serovar hardjo by ribotyping, arbitrarily primed PCR, and mapped restriction site polymorphisms
نویسندگان
چکیده
منابع مشابه
molecular characterization of epidemic isolates of vibrio cholerae o1 by arbitrarily primed pcr (ap-pcr)
background: epidemic and endemic cholera is a major public health problem for many countries. aim of this study was to evaluate ap-pcr for investigation of clonal relatedness among the strains of vibrio cholerae recovered from an outbreak occurred in different parts of iran in 2005. methods: the study was conducted during the cholera outbreak occurred in some of provinces in iran in summer 2...
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Clostridium difficile is now recognized as the major agent responsible for nosocomial diarrhea in adults. Among the genotyping methods available, arbitrarily primed PCR (AP-PCR), PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) have been widely used for investigating outbreaks of C. difficile infections. However, the comparative typing ability, reproducibility, discriminatory power, ...
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BACKGROUND Leptospirosis is caused by pathogenic spirochetes of the genus Leptospira spp. This zoonotic disease is distributed globally and affects domestic animals, including cattle. Leptospira interrogans serogroup Sejroe serovar Hardjo and Leptospira borgpetersenii serogroup Sejroe serovar Hardjo remain important species associated with this reproductive disease in livestock production. Prev...
متن کاملAnalysis of Leptospira isolates from mainland Portugal and the Azores islands.
From 228 recent Leptospira isolates from mainland Portugal and Azorean wild mammals, 149 were characterized at the serovar level by monoclonal antibodies (MAbs), a quick serological method in epidemiological studies. In order to compare this antigenic information with that from new genetic techniques, a sample of isolates was analyzed through pulsed-field agarose gel electrophoresis (PFGE) (n=7...
متن کاملIdentification and characterization of the protein antigens of Leptospira interrogans serovar hardjo.
We radiolabeled Leptospira proteins with [35S]methionine. Solubilized extracts of radiolabeled L. interrogans serovar hardjo strain hardjoprajitno were analyzed by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography. We compared the protein profile obtained in this manner to the protein profiles of various [35S]methionine-labeled Leptospira spp. The profil...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1994
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.32.8.1949-1957.1994